Release and subcellular localization of acidic fibroblast growth factor expressed to high levels in HeLa cells.

Acidic fibroblast growth factor (aFGF) lacks a classical signal sequence for secretion via the exocytic pathway but yet has to be released from cells in order to interact with high affinity receptors on the cell surface. To study the release process, we have expressed human aFGF in HeLa cells using a T7 RNA polymerase-driven vaccinia virus system. The high… CONTINUE READING