Relaxation of a single DNA molecule observed by optical microscopy.

@article{Perkins1994RelaxationOA,
  title={Relaxation of a single DNA molecule observed by optical microscopy.},
  author={Thomas T. Perkins and Stephen R. Quake and David Eugene Smith and Steven Chu},
  journal={Science},
  year={1994},
  volume={264 5160},
  pages={
          822-6
        }
}
Single molecules of DNA, visualized in video fluorescence microscopy, were stretched to full extension in a flow, and their relaxation was measured when the flow stopped. The molecules, attached by one end to a 1-micrometer bead, were manipulated in an aqueous solution with optical tweezers. Inverse Laplace transformations of the relaxation data yielded spectra of decaying exponentials with distinct peaks, and the longest time component (tau) increased with length (L) as tau approximately L 1… Expand
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