Relative replicative fitness of zidovudine-resistant human immunodeficiency virus type 1 isolates in vitro.

@article{Harrigan1998RelativeRF,
  title={Relative replicative fitness of zidovudine-resistant human immunodeficiency virus type 1 isolates in vitro.},
  author={P. Richard Harrigan and Stuart Bloor and Brendan Larder},
  journal={Journal of virology},
  year={1998},
  volume={72 5},
  pages={3773-8}
}
Replication of mixtures of two or more human immunodeficiency virus type 1 (HIV-1) variants would be expected to result in the eventual selection of the fittest virus due to Darwinian competition among the variants. The relative proportions of known HIV-1 variants (which may differ only by a single nucleotide from a standard "wild-type" virus, HIV-1HXB2) in mixed viral cultures were quantified by analysis of automated sequence signals of reverse transcriptase PCR products. With this method, the… CONTINUE READING