Relative quantitation of protein nitration by liquid chromatography-mass spectrometry using isotope-coded dimethyl labeling and chemoprecipitation.

@article{Guo2012RelativeQO,
  title={Relative quantitation of protein nitration by liquid chromatography-mass spectrometry using isotope-coded dimethyl labeling and chemoprecipitation.},
  author={Jia Guo and Katalin Prokai-Tatrai and Laszlo Prokai},
  journal={Journal of chromatography. A},
  year={2012},
  volume={1232},
  pages={266-75}
}
Protein nitration has been recognized as an important biomarker for nitroxidative stress associated with various diseases. While identification of protein targets for nitration is important, its quantitative profiling also is necessary to understand the biological impact of this low-abundance posttranslational modification. We have previously reported an efficient and straightforward enrichment method for nitropeptides to reduce sample complexity and permit unambiguous site-specific… CONTINUE READING