Relative quantification of angiotensin-converting enzyme mRNA in human smooth muscle cells, monocytes, and lymphocytes by the polymerase chain reaction.

@article{Aschoff1994RelativeQO,
  title={Relative quantification of angiotensin-converting enzyme mRNA in human smooth muscle cells, monocytes, and lymphocytes by the polymerase chain reaction.},
  author={J M Aschoff and D S Lazarus and Barry L. Fanburg and Joseph J. Lanzillo},
  journal={Analytical biochemistry},
  year={1994},
  volume={219 2},
  pages={218-23}
}
A method was developed for relative quantification of angiotensin-converting enzyme (ACE) mRNA in as few as 100 cells. After reverse transcription of total RNA to cDNA, multiplexed polymerase chain reaction with two sets of primers amplified ACE cDNA and that of an internal standard glyceraldehyde phosphate dehydrogenase (GAPDH) simultaneously. By adjusting… CONTINUE READING