Relative quantification: characterization of bias, variability and fold changes in mass spectrometry data from iTRAQ-labeled peptides.

@article{Mahoney2011RelativeQC,
  title={Relative quantification: characterization of bias, variability and fold changes in mass spectrometry data from iTRAQ-labeled peptides.},
  author={Douglas W. Mahoney and Terry M. Therneau and Carrie J. Heppelmann and LeeAnn Higgins and Linda M. Benson and Roman Zenka and Pratik Jagtap and Gary L. Nelsestuen and H. R. Bergen and Ann L. Oberg},
  journal={Journal of proteome research},
  year={2011},
  volume={10 9},
  pages={4325-33}
}
Shotgun proteomics via mass spectrometry (MS) is a powerful technology for biomarker discovery that has the potential to lead to noninvasive disease screening mechanisms. Successful application of MS-based proteomics technologies for biomarker discovery requires accurate expectations of bias, reproducibility, variance, and the true detectable differences in platforms chosen for analyses. Characterization of the variability inherent in MS assays is vital and should affect interpretation of… CONTINUE READING