Relative orientation of RNA helices in a group 1 ribozyme determined by helix extension electron microscopy.

@article{Nakamura1995RelativeOO,
  title={Relative orientation of RNA helices in a group 1 ribozyme determined by helix extension electron microscopy.},
  author={Toru M Nakamura and Ying Hong Wang and Arthur J. Zaug and Jack D. Griffith and Thomas R Cech},
  journal={The EMBO journal},
  year={1995},
  volume={14 19},
  pages={4849-59}
}
The relative orientation of helical elements in a folded RNA molecule provides key information about its three-dimensional architecture. We have developed a method that involves extending peripheral helices of an RNA, mounting for electron microscopy in the absence of protein and measuring interhelical angles. As a control, extended anticodon and acceptor… CONTINUE READING