Relative mitogenic activities of wild-type and retinoblastoma binding-defective SV40 T antigens in serum-deprived and senescent human diploid fibroblasts.

@article{Sakamoto1993RelativeMA,
  title={Relative mitogenic activities of wild-type and retinoblastoma binding-defective SV40 T antigens in serum-deprived and senescent human diploid fibroblasts.},
  author={Kentaro Sakamoto and Thomas H. Howard and Vasily V. Ogryzko and Naidi Xu and C C Corsico and D. Holstead Jones and Bruce H Howard},
  journal={Oncogene},
  year={1993},
  volume={8 7},
  pages={
          1887-93
        }
}
A novel gene transfer approach was used to investigate whether the retinoblastoma (Rb)-binding domain of simian virus 40 (SV40) T antigen is required for efficient T antigen-mediated stimulation of DNA synthesis is quiescent or senescent human embryo fibroblasts. In senescent cells, comparison between wild-type T antigen and a mutant defective in Rb binding (Glu-107-->Lys) revealed the latter to have approximately 15-fold lower activity. In contrast, comparison of wild-type and Rb- T antigens… CONTINUE READING