Relative abundance of Bacteroides spp. in stools and wastewaters as determined by hierarchical oligonucleotide primer extension.

@article{Hong2008RelativeAO,
  title={Relative abundance of Bacteroides spp. in stools and wastewaters as determined by hierarchical oligonucleotide primer extension.},
  author={Pei-Ying Hong and Jer-Horng Wu and Wen-Tso Liu},
  journal={Applied and environmental microbiology},
  year={2008},
  volume={74 9},
  pages={2882-93}
}
A molecular method, termed hierarchical oligonucleotide primer extension (HOPE), was used to determine the relative abundances of predominant Bacteroides spp. present in fecal microbiota and wastewaters. For this analysis, genomic DNA in feces of healthy human adults, bovines, and swine and in wastewaters was extracted and total bacterial 16S rRNA genes were PCR amplified and used as the DNA templates for HOPE. Nineteen oligonucleotide primers were designed to detect 14 Bacteroides spp. at… CONTINUE READING
22 Citations
42 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 22 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 42 references

16S/23S rRNA sequencing, p. 115–175

  • D. J. Lane
  • 1991
Highly Influential
2 Excerpts

Similar Papers

Loading similar papers…