The structure of humor expression was clarified and its relationships with aggression, altruism, and self-acceptance were examined. In study 1, college students (n = 216) responded to a scale with items about humor expression. An exploratory factor analysis indicated three types of humor expression: aggressive, self-disparaging, and playful humor expression. In study 2, 119 college students responded to items about (a) humor expression, (b) aggression, (c) altruism, and (d) self-acceptance. The results showed positive relationships between aggressive humor expression and aggression, self-disparaging humor expression and self-acceptance, and playful humor expression and altruism.