Relationship of proton release at the extracellular surface to deprotonation of the schiff base in the bacteriorhodopsin photocycle.

@article{Cao1995RelationshipOP,
  title={Relationship of proton release at the extracellular surface to deprotonation of the schiff base in the bacteriorhodopsin photocycle.},
  author={Y Cao and Leonid S. Brown and Jun Sasaki and Akio Maeda and Richard Needleman and Janos K. Lanyi},
  journal={Biophysical journal},
  year={1995},
  volume={68 4},
  pages={1518-30}
}
The surface potential of purple membranes and the release of protons during the bacteriorhodopsin photocycle have been studied with the covalently linked pH indicator dye, fluorescein. The titration of acidic lipids appears to cause the surface potential to be pH-dependent and causes other deviations from ideal behavior. If these anomalies are neglected, the appearance of protons can be followed by measuring the absorption change of fluorescein bound to various residues at the extracellular… CONTINUE READING