Relationship of human liver dihydrodiol dehydrogenases to hepatic bile-acid-binding protein and an oxidoreductase of human colon cells.

@article{Hara1996RelationshipOH,
  title={Relationship of human liver dihydrodiol dehydrogenases to hepatic bile-acid-binding protein and an oxidoreductase of human colon cells.},
  author={Akira Hara and Kazuya Matsuura and Yoshiyuki Tamada and Kiyomi Sato and Yoshiyuki Miyabe and Yoshihiro Deyashiki and Norihisa Ishida},
  journal={The Biochemical journal},
  year={1996},
  volume={313 ( Pt 2)},
  pages={373-6}
}
We previously isolated three monomeric dihydrodiol dehydrogenases, DD1, DD2 and DD4, from human liver, and cloned a cDNA (C9) thought to encode DD2, which is identical with those for human bile-acid-binding protein and an oxidoreductase of human colon carcinoma HT29 cells. In the present study we have provided evidence that the C9 cDNA clone encodes DD1, not DD2. A recombinant enzyme expressed from the cDNA in a bacterial system was purified, and its catalytic properties, bile-acid-binding… CONTINUE READING