Relationship between tissue plasminogen activator production and specific growth rate in Chinese Hamster Ovary cells cultured in mannose at low temperature

@article{Berros2009RelationshipBT,
  title={Relationship between tissue plasminogen activator production and specific growth rate in Chinese Hamster Ovary cells cultured in mannose at low temperature},
  author={Julio Berr{\'i}os and Alvaro D{\'i}az-Barrera and Consuelo Baz{\'a}n and Claudia Altamirano},
  journal={Biotechnology Letters},
  year={2009},
  volume={31},
  pages={1493-1497}
}
Chinese hamster ovary (CHO) cells, producing human recombinant tissue plasminogen activator (tPA), were grown with mannose (5, 20 and 40 mM) instead of glucose at 31, 33 and 37°C. The highest tPA concentration (1.5 mg l−1 at 144 h of cultivation) and tPA specific production rate (47 ng 10−6 cell h−1) were obtained at 31°C and 40 mM mannose. Regardless of the temperature or mannose concentration used, an inverse relationship between the specific growth rate and tPA specific production rate was… CONTINUE READING

References

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Mannose as carbon source improves productivity and sialic acid content of tPA produced by CHO cells in continuous cultures

  • J Berrios, C Altamirano
  • 2009

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