Relationship between stability and function for isolated domains of troponin C.

Abstract

Results of spectroscopic thermal and chemical denaturation studies and calcium binding studies are presented for a series of five recombinant chicken troponin C fragments. They were designed to assess the effects of domain isolation, N-helix, and D/E linker helix on stability and calcium affinity. Four of the fragments include the N-terminal regulatory… (More)

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