Relationship between membrane physical properties and secretory phospholipase A2 hydrolysis kinetics in S49 cells during ionophore-induced apoptosis.

@article{Bailey2007RelationshipBM,
  title={Relationship between membrane physical properties and secretory phospholipase A2 hydrolysis kinetics in S49 cells during ionophore-induced apoptosis.},
  author={Rachel W Bailey and Erin D Olson and Mai P Vu and Taylor J Brueseke and Leslie Robertson and Ryan E Christensen and Kristen H Parker and Allan M. Judd and John D. Bell},
  journal={Biophysical journal},
  year={2007},
  volume={93 7},
  pages={2350-62}
}
During apoptosis, changes occur in lymphocyte membranes that render them susceptible to hydrolysis by secretory phospholipase A(2) (sPLA(2)). To study the relevant mechanisms, a simplified model of apoptosis using a calcium ionophore was applied. Kinetic and flow cytometry experiments provided key observations regarding ionophore treatment: the initial rate of hydrolysis was elevated at all enzyme concentrations, the total amount of reaction product was increased fourfold, and adsorption of the… CONTINUE READING

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