Relationship between in vivo activity and in vitro measures of function and stability of a protein.

@article{Sandberg1995RelationshipBI,
  title={Relationship between in vivo activity and in vitro measures of function and stability of a protein.},
  author={Warren S Sandberg and P M Schlunk and H B Zabin and Thomas C. Terwilliger},
  journal={Biochemistry},
  year={1995},
  volume={34 37},
  pages={11970-8}
}
The in vivo activities of mutant proteins are readily measured and can potentially be used to estimate changes in in vitro properties such as stability or function, but this connection has not been rigorously established. Gene V protein is a small protein produced by bacteriophage f1 that binds to single-stranded DNA and to RNA and for which fitness can be assayed both in vivo and in vitro. We have assembled a large number of temperature-sensitive mutants of the gene V protein of bacteriophage… CONTINUE READING