Relationship between NAD(P)H:quinone oxidoreductase 1 (NQO1) levels in a series of stably transfected cell lines and susceptibility to antitumor quinones.

@article{Winski2001RelationshipBN,
  title={Relationship between NAD(P)H:quinone oxidoreductase 1 (NQO1) levels in a series of stably transfected cell lines and susceptibility to antitumor quinones.},
  author={Shannon L Winski and Elizabeth G. Swann and Robert H J Hargreaves and Donna L Dehn and John L Butler and Christopher J Moody and David D. Ross},
  journal={Biochemical pharmacology},
  year={2001},
  volume={61 12},
  pages={1509-16}
}
To investigate the importance of NAD(P)H:quinone oxidoreductase 1 (or DT-diaphorase; NQO1) in the bioactivation of antitumor quinones, we established a series of stably transfected cell lines derived from BE human colon adenocarcinoma cells. BE cells have no NQO1 activity due to a genetic polymorphism. The new cell lines, BE-NQ, stably express wild-type NQO1. BE-NQ7 cells expressed the highest level of NQO1 and were more susceptible [determined by the thiazolyl blue (MTT) assay] to known… CONTINUE READING

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