Relationship between DNA damage response, initiated by camptothecin or oxidative stress, and DNA replication, analyzed by quantitative 3D image analysis.

@article{Berniak2013RelationshipBD,
  title={Relationship between DNA damage response, initiated by camptothecin or oxidative stress, and DNA replication, analyzed by quantitative 3D image analysis.},
  author={Krzysztof Berniak and Paulina Rybak and Tytus Bernaś and Mirosław A Zarebski and Ewa Biela and Haishuang Zhao and Zbigniew Darzynkiewicz and Jerzy W. Dobrucki},
  journal={Cytometry. Part A : the journal of the International Society for Analytical Cytology},
  year={2013},
  volume={83 10},
  pages={
          913-24
        }
}
A method of quantitative analysis of spatial (3D) relationship between discrete nuclear events detected by confocal microscopy is described and applied in analysis of a dependence between sites of DNA damage signaling (γH2AX foci) and DNA replication (EdU incorporation) in cells subjected to treatments with camptothecin (Cpt) or hydrogen peroxide (H2O2). Cpt induces γH2AX foci, likely reporting formation of DNA double-strand breaks (DSBs), almost exclusively at sites of DNA replication. This… CONTINUE READING
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