Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification?

@article{GallardoEscrate2007RelationshipBD,
  title={Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification?},
  author={Cristi{\'a}n Gallardo-Esc{\'a}rate and Josu{\'e} {\'A}lvarez-Borrego and Elisabeth Von Brand and Enrique M Dupr{\'e} and Miguel {\'A}ngel Del R{\'i}o-Portilla},
  journal={Biological research},
  year={2007},
  volume={40 1},
  pages={29-40}
}
In observations by confocal or conventional fluorescence microscopy, important factors should be considered in order to obtain accurate images. One of them, such as the fluorescence bleaching from highest intensity to lowest signal of fluorescence is a common problem with several DNA fluorochromes and especially for DAPI stain. The fluorescence of DAPI fades rapidly when it is exposed to UV light, under optimal conditions of observation. Although the fading process can be retarded using a… CONTINUE READING