Relation of Protein Synthesis to the Division Cycle in Mammalian Cell Cultures


Protein synthesis in suspension cultures of human cells in logarithmic growth was inhibited with puromycin or chloramphenicol, and the growth rate and mitotic index were measured as a function of time. The mitotic index remained constant for about 1 hour after addition of inhibitor; this indicates that any protein synthesis necessary for mitosis is… (More)


5 Figures and Tables