Relation between Pyridine Nucleotide-dependent Dehydrogenase Activity and Nicotinamide Adenine Dinucleotide Glycohydrolase in Ehrlich Ascites Tumor Cells

The crystalline form of the glycolytic enzyme G3PDH2 from rabbit muscle may contain up to 4 moles NAD+/mole enzyme protein (29) and requires this NAD@ for the stabii zation of its structure and for protection against proteolytic inactivation by trypsin (1 1, 36). We have previously shown that the rate at which G3PDH-bound NAD@ was hydrolyzed was 20% that of… CONTINUE READING