Reinforced Cytotoxicity of Lymphokine‐activated Killer Cells toward Glioma Cells by Transfection of the Killer Cells with the γ‐Interferon Gene

Lymphokine-activated killer (LAK) cells generated from peripheral blood lymphocytes incubated with recombinant interleukin-2 were transfected with the human gamma-interferon (HuIFN-gamma) gene by means of liposomes having a positive charge on their surface. The cells secreted significant amounts of HuIFN-gamma (reaching more than 5 U/ml) into the culture… CONTINUE READING