Regulatory volume increase after hypertonicity- or vasoactive-intestinal-peptide-induced cell-volume decrease in small-intestinal crypts is dependent on Na+-K+-2Cl− cotransport

@article{Obrien1993RegulatoryVI,
  title={Regulatory volume increase after hypertonicity- or vasoactive-intestinal-peptide-induced cell-volume decrease in small-intestinal crypts is dependent on Na+-K+-2Cl− cotransport},
  author={Julie A O'brien and Rhodri J. Walters and M. A. Lopez Valverde and Francisco V Sep{\'u}lveda},
  journal={Pfl{\"u}gers Archiv},
  year={1993},
  volume={423},
  pages={67-73}
}
The volume of intact crypts isolated from guinea-pig small intestine has been measured to assess the capacity of the cells to regulate their volume after hypertonic shock or vasoactive-intestinal-peptide (VIP)-induced shrinkage. Crypts exposed to anisotonic medium initially behave as perfect osmometers. Continued exposure to a hypertonic (400 mosmol/l) medium was followed by regulatory volume increase (RVI), which led to complete volume recovery in about 20 min. VIP produced a volume reduction… CONTINUE READING