Regulatory role of translocation of Na+-K+ pumps in skeletal muscle: hypothesis or reality?

  title={Regulatory role of translocation of Na+-K+ pumps in skeletal muscle: hypothesis or reality?},
  author={Torben Clausen},
  journal={American journal of physiology. Endocrinology and metabolism},
  volume={295 3},
          E727-8; author reply 729
  • T. Clausen
  • Published 1 September 2008
  • Biology
  • American journal of physiology. Endocrinology and metabolism
to the editor: The Perspectives article by Benziane and Chibalin ([1][1]) in this issue comments on part of the literature regarding the possible role of translocation in the regulation of Na+-K+ pumps in skeletal muscle. I have been invited to submit a letter to the editor about this review article 

Na,K-ATPase regulation in skeletal muscle.

This review focuses on molecular mechanisms that underlie regulation of NKA in skeletal muscle by major extrinsic and local stimuli, such as insulin and the energy-sensing AMP-activated protein kinase, and the recently uncovered roles for glutathionylation, nitric oxide, and extracellular K(+) in the regulation.

Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart

It is shown by immunofluorescence and co-immunoprecipitation that α2 is preferentially expressed with β2 in T-tubules of cardiac myocytes, forming α2β2 heterodimers, which could allow an acute response to raised ambient K+ concentrations in physiological conditions and explain the importance of α2 β2 for cardiac muscle contractility.


This research applied microelectrode arrays to record the spontaneous sinoatrial node field potentials of C57/BL6J mice and analyzed the effects of different glucose concentrations in time domain and frequency domain by using statistical method, vector maps and fast Fourier transform.

Insight into miRNAs related with glucometabolic disorder.

Reply to Clausen letter

Recent developments in the field of skeletal muscle Na+-K+-ATPase regulation are highlighted and how this builds on the important earlier data highlighted in this article.



Frontiers: skeletal muscle sodium pump regulation: a translocation paradigm.

The skeletal muscle sodium pump plays a major role in the removal of K(+) ions from the circulation postprandial, or after a physical activity bout, thereby preventing the development of hyperkalemia

Active Na—K transport and the rate of ouabain binding. The effect of insulin and other stimuli on skeletal muscle and adipocytes

The effect of stimulation or inhibition of active Na—K transport on [3H]ouabain binding has been investigated in isolated soleus muscles and adipocytes and the results confirmed the importance of knowing the carrier and removal status of Na-K in the transport of H2O.

Na+-K+ pump regulation and skeletal muscle contractility.

  • T. Clausen
  • Biology, Medicine
    Physiological reviews
  • 2003
The Na+-K+ pump is a central target for regulation of Na-k+ distribution and excitability, essential for second-to-second ongoing maintenance of excitability during work.

The number of sodium ion pumping sites in skeletal muscle and its modification by insulin.

It is concluded that there are two pools of Na pumping sites in muscle cells: one active and another inactive, and insulin unmasks the inactive pumping sites by a mechanism that is independent of protein synthesis, increases in intracellular [Na] or decreases in intrACEllular [K].

Quantitative determination of Na+-K+-ATPase and other sarcolemmal components in muscle cells.

Recent studies have shown that regulatory changes in the entire population of Na+-K+ pumps in muscle can be quantified in measurements of [3H]-ouabain binding, K+-activated 3-O-methylfluorescein phosphatase activity, as well as maximum ouabain suppressible Na-k+ transport capacity.

Ouabain binding and Na+K+ transport in rat muscle cells and adipocytes

AMPK activation with AICAR provokes an acute fall in plasma [K+].

It is illustrated that activation of AMPK activity with AICAR provokes a significant fall in plasma [K(+)] and a novel mechanism for redistributing K(+) from ECF to ICF is suggested.

The effect of insulin on the transport of sodium and potassium in rat soleus muscle.

It is concluded that insulin stimulates the active coupled transport of Na and K, possibly by increasing the relative Na‐affinity of the system mediating this process.

Analysis of exercise‐induced Na+–K+ exchange in rat skeletal muscle in vivo

In the soleus, in vivo exercise induces a rise in intracellular Na+, which reflects the excitation‐induced increase in Na+ influx and leads to augmented Na+–K+ pump activity without apparent change in Na+, and there was no effect of exercise on [3H]ouabain binding measured in vitro or in vivo.

Cardiotonic Steroids Stimulate Glycogen Synthesis in Human Skeletal Muscle Cells via a Src- and ERK1/2-dependent Mechanism*

Ouabain and marinobufagenin stimulate glycogen synthesis in skeletal muscle cells and this effect is mediated by activation of a Src-, ERK1/2, p90rsk-, and GSK3-dependent signaling pathway.