Regulatory properties of rat heart AMP deaminase.

  title={Regulatory properties of rat heart AMP deaminase.},
  author={Krystian K. Kaletha and Andrzej C. Składanowski},
  journal={Biochimica et biophysica acta},
  volume={568 1},
The kinetic and regulatory properties of purified rat heart AMP deaminase were investigated. In the presence of 100 mM KCl, the enzyme exhibited a slightly sigmoid-shaped plot of reaction rate, vs. substrate concentration, which shifted to a more hyperbolic form when ATP, ADP or GTP were added. ATP was the most potent activator of the enzyme, whereas GTP at low (less than 0.25 mM) concentrations increased the enzyme activity. The activation effect was negligible at higher concentrations of GTP… CONTINUE READING