Regulatory features of transcription in isolated mitochondria from Artemia franciscana embryos.

  title={Regulatory features of transcription in isolated mitochondria from Artemia franciscana embryos.},
  author={Brian Donovan Eads and S. C. Hand},
  journal={American journal of physiology. Regulatory, integrative and comparative physiology},
  volume={277 6},
Optimal conditions were developed for an in organello transcriptional run-on assay using mitochondria isolated from Artemia franciscana embryos to investigate potential regulatory features of RNA synthesis under conditions of anoxia-induced quiescence. Transcription is not dependent on oxidative phosphorylation for maximal activity when exogenous ATP is available. Bona fide transcription products, as assessed by hybridization with specific mitochondrial cDNAs from A. franciscana, are produced… CONTINUE READING
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