Regulatory circuits of the AAA+ disaggregase Hsp104.

  title={Regulatory circuits of the AAA+ disaggregase Hsp104.},
  author={Titus M. Franzmann and Anna Czekalla and Stefan G Walter},
  journal={The Journal of biological chemistry},
  volume={286 20},
Yeast Hsp104 is an AAA+ chaperone that rescues proteins from the aggregated state. Six protomers associate to form the functional hexamer. Each protomer contains two AAA+ modules, NBD1 and NBD2. Hsp104 converts energy provided by ATP into mechanical force used to thread polypeptides through its axial channel, thereby disrupting protein aggregates. But how the action of its 12 AAA+ domains is co-ordinated to catalyze disaggregation remained unexplained. Here, we identify a sophisticated… CONTINUE READING