Regulatory C Protein of the EcoRV Modification–Restriction System

  title={Regulatory C Protein of the EcoRV Modification–Restriction System},
  author={Ludmila A Zheleznaya and Denis E Kainov and Alfiya K Yunusova and Nicholas I Matvienko},
  journal={Biochemistry (Moscow)},
The C gene product of the modification–restriction system PvuII binds to its own promoter (C box) and stimulates transcription of both the C gene and the endonuclease gene. According to our data the same regulatory mechanism is realized in the EcoRV system. It was found that upstream of the EcoRV endonuclease gene two ATG codons give rise to two open reading frames (ORF1 and ORF2) ending at the same point inside the endonuclease gene. Two DNA fragments corresponding to ORF1 and ORF2 were cloned… CONTINUE READING