Regulation through the Secondary Channel—Structural Framework for ppGpp-DksA Synergism during Transcription

@article{Perederina2004RegulationTT,
  title={Regulation through the Secondary Channel—Structural Framework for ppGpp-DksA Synergism during Transcription},
  author={Anna A Perederina and Vladimir Svetlov and Marina N. Vassylyeva and Tahir H. Tahirov and Shigeyuki Yokoyama and Irina Artsimovitch and Dmitry G. Vassylyev},
  journal={Cell},
  year={2004},
  volume={118},
  pages={297-309}
}
Bacterial transcription is regulated by the alarmone ppGpp, which binds near the catalytic site of RNA polymerase (RNAP) and modulates its activity. We show that the DksA protein is a crucial component of ppGpp-dependent regulation. The 2.0 A resolution structure of Escherichia coli DksA reveals a globular domain and a coiled coil with two highly conserved Asp residues at its tip that is reminiscent of the transcript cleavage factor GreA. This structural similarity suggests that DksA coiled… CONTINUE READING
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