Regulation of vitamin D-1alpha-hydroxylase in a human cortical collecting duct cell line.

@article{Bland2001RegulationOV,
  title={Regulation of vitamin D-1alpha-hydroxylase in a human cortical collecting duct cell line.},
  author={Rosemary Bland and D. Zehnder and Susan V. Hughes and Pierre Ronco and P. M. Stewart and Martin Hewison},
  journal={Kidney international},
  year={2001},
  volume={60 4},
  pages={1277-86}
}
BACKGROUND Recent studies have shown that renal expression of 25-hydroxyvitamin D3-1alpha-hydroxylase (1alpha-OHase) is not restricted to proximal tubules. To investigate the significance of this expression, we characterized the regulation of 1alpha-OHase expression and activity in a human cortical collecting duct cell line (HCD). METHODS Expression of 1alpha-OHase mRNA and protein was assessed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analyses. Enzyme… CONTINUE READING