Regulation of thymidylate synthetase in mouse leukemia cells (L1210).

  title={Regulation of thymidylate synthetase in mouse leukemia cells (L1210).},
  author={Wojciech Rode and Kevin J. Scanlon and Barbara A. Moroson and Joseph R. Bertino},
  journal={The Journal of biological chemistry},
  volume={255 4},
L1210 mouse leukemia cells were synchronized by isoleucine deprivation followed by hydroxyurea treatment. Changes of thymidylate synthetase activity both in cell extracts and in the intact cells (in situ) were followed throughout the cell cycle. The enzyme activity assayed in situ, by measuring tritium released from [5-3H]deoxyuridine, changed markedly in relation to the DNA synthesis pattern, whereas that assayed in cell extracts remained unchanged. In the S phase of the cell cycle, activity… CONTINUE READING