Regulation of thrombopoietin levels by c-mpl-mediated binding to platelets.

  title={Regulation of thrombopoietin levels by c-mpl-mediated binding to platelets.},
  author={Paul J. Fielder and Austin L. Gurney and Eric G. Stefanich and Mary Marian and Mark W. Moore and Karen Carver-Moore and Frederic J de Sauvage},
  volume={87 6},
The involvement of platelets and the c-mpl receptor in the regulation of thrombopoietin (TPO) plasma concentrations and tissue mRNA levels was investigated in both normal mice and mice defective in c-mpl (c-mpl-/-). Although c-mpl-/- mice have fewer platelets and higher plasma TPO activity than normal mice, there was no increase in TPO mRNA levels as measured by an S1 nuclease protection assay. After the intravenous injection of 125I-TPO, specific uptake of radioactivity by the spleen and blood… CONTINUE READING