Regulation of the sodium-potassium pump in cultured rat skeletal myotubes by intracellular sodium ions.

The properties of the Na-K pump and some of the factors controlling its amount and function were studied in rat myotubes in culture. The number of Na-K pump sites was quantified by measuring the amount of [3H]ouabain bound to whole-cell preparations. Activity of the pump was determined by measurement of ouabain-sensitive 86Rb-uptake and component of… (More)