Regulation of the display ratio of enzymes on the Saccharomyces cerevisiae cell surface by the immunoglobulin G and cellulosomal enzyme binding domains.

@article{Ito2009RegulationOT,
  title={Regulation of the display ratio of enzymes on the Saccharomyces cerevisiae cell surface by the immunoglobulin G and cellulosomal enzyme binding domains.},
  author={Junji Ito and Akihiko Kosugi and Tsutomu Tanaka and Kouichi Kuroda and Seiji Shibasaki and Chiaki Ogino and Mitsuyoshi Ueda and Hideki Fukuda and Roy H. Doi and Akihiko Kondo},
  journal={Applied and environmental microbiology},
  year={2009},
  volume={75 12},
  pages={4149-54}
}
We constructed a novel cell surface display system to control the ratio of target proteins on the Saccharomyces cerevisiae cell surface, using two pairs of protein-protein interactions. One protein pair is the Z domain of protein A derived from Staphylococcus aureus and the Fc domain of human immunoglobulin G. The other is the cohesin (Coh) and dockerin (Dock) from the cellulosome of Clostridium cellulovorans. In this proposed displaying system, the scaffolding proteins (fusion proteins of Z… CONTINUE READING