Regulation of the activity of the transcription factor Runx2 by two homeobox proteins, Msx2 and Dlx5

@article{Shirakabe2001RegulationOT,
  title={Regulation of the activity of the transcription factor Runx2 by two homeobox proteins, Msx2 and Dlx5},
  author={Kyoko Shirakabe and Kazuya Terasawa and K Miyama and Hiroshi Shibuya and Eisuke Nishida},
  journal={Genes to Cells},
  year={2001},
  volume={6}
}
Background Runx2, formerly called PEBP2αA or Cbfa1, is a transcription factor whose deletion causes a complete lack of ossification. It directly regulates the expression of osteoblast‐specific genes through the osteoblast‐specific cis‐acting element found in the promoter region of these genes. 
Role and regulation of RUNX2 in osteogenesis.
TLDR
The regulation of RUNX2 by factors commonly used during osteogenic studies will be discussed and the complexity of its regulation and its interactions within the osteoblast differentiation pathway are often overlooked.
RUNX family: Regulation and diversification of roles through interacting proteins
TLDR
The abilities of RUNX proteins, in particular RUNX3, to integrate oncogenic signals or environmental cues and to initiate appropriate tumor suppressive responses are discussed.
Craniosynostosis‐Associated Gene Nell‐1 Is Regulated by Runx2
TLDR
Three OSE2 elements in the NELL‐1 promoter are identified that are directly bound and transactivated by Runx2, a craniosynostosis‐related gene that is involved in transcriptional regulation in rat calvarial cells.
Advances in Runx2 regulation and its isoforms.
Transcription factors controlling osteoblastogenesis.
  • P. Marie
  • Biology
    Archives of biochemistry and biophysics
  • 2008
Cooperative Interactions between RUNX2 and Homeodomain Protein-binding Sites Are Critical for the Osteoblast-specific Expression of the Bone Sialoprotein Gene*
TLDR
The data show that RUNX2 is a direct regulator of Bsp in osteoblasts and that it functions in cooperation with DLX5 or a related factor to activate osteoblast-specific gene expression.
Dlx5 Specifically Regulates Runx2 Type II Expression by Binding to Homeodomain-response Elements in the Runx2 Distal Promoter*
TLDR
Dlx5 specifically transactivates the Runx2 P1 promoter, and its action on the P1 promoters is antagonized by Msx2, and the responsible region overlaps with that recognized by Dlx 5.
Loss of Smad3-Mediated Negative Regulation of Runx2 Activity Leads to an Alteration in Cell Fate Determination
TLDR
It is reported that primary mouse dermal fibroblasts lacking Smad3 can acquire an osteoblast-like phenotype, including activation of Runx2 activity, expression of osteobasts-specific genes, and calcium deposition, and negative regulation of Run x2 activity by Smad 3 in dermal Fibroblast is likely mediated by controlling the expression of Msx2, an antagonist of RunX2 in this cellular context.
The suppressive effect of myeloid elf‐1‐like factor (MEF) in osteogenic differentiation
TLDR
It is suggested that MEF promotes cell proliferation and functions as a negative regulator of osteogenic differentiation by directly interacting with Runx2 and suppressing its transcriptional activity.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 33 REFERENCES
Molecular basis of tissue‐specific gene expression mediated by the Runt domain transcription factor PEBP2/CBF
  • Yoshiaki Ito
  • Biology
    Genes to cells : devoted to molecular & cellular mechanisms
  • 1999
TLDR
A unique functional interaction between PEBP2αB/AML1 and Ets‐1 was recently observed, and provides a clear example of context‐dependent transcriptional regulation.
Two distinct osteoblast-specific cis-acting elements control expression of a mouse osteocalcin gene
TLDR
It is demonstrated that two distinct cis-acting elements are responsible for osteoblast expression of mouse osteocalcin gene 2 and provides for the first time a functional characterization of osteooblast-specific cis- acting elements.
Stimulus-selective Inhibition of Rat Osteocalcin Promoter Induction and Protein-DNA Interactions by the Homeodomain Repressor Msx2*
TLDR
Msx2 abrogates up-regulation of the OC promoter by FGF2/FSK in part by inhibiting O CFREB binding to the OCFRE, and compared these interactions with those occurring at the calcitriol response element (VDRE).
A PEBP2/AML-1-related Factor Increases Osteocalcin Promoter Activity through Its Binding to an Osteoblast-specific cis-Acting Element (*)
TLDR
This study demonstrates that AML-1B can increase gene expression of an osteoblast-specific gene through its binding to an osteo-specific cis-acting element and presents evidence that OSF2 is a member of the PEBP2α/AML- 1 family of transcription factors.
Heterodimerization of Msx and Dlx homeoproteins results in functional antagonism.
TLDR
It is proposed that functional antagonism through heterodimer formation provides a mechanism for regulating the transcriptional actions of Msx and Dlx homeoproteins in vivo.
Missense mutations abolishing DNA binding of the osteoblast-specific transcription factor OSF2/CBFA1 in cleidocranial dysplasia
TLDR
DNA-binding studies with the mutant polypeptides show that these amino acid substitutions abolish the DNA-binding ability of OSF2/CBFA1 to its known target sequence, and direct genetic evidence that the phenotype is secondary to an alteration of osteoblast differentiation is provided.
Msx-2/Hox 8.1: a transcriptional regulator of the rat osteocalcin promoter.
TLDR
Examination of rodent osteoblastic cells lines for expression of Msx homeodomain-encoding messages suggests that Msx-2 may play a role in the transcriptional regulation of the osteoblast phenotype during development in the morphogenetic fields where it is expressed.
Mutations Involving the Transcription Factor CBFA1 Cause Cleidocranial Dysplasia
...
1
2
3
4
...