Regulation of the Escherichia coli L-arabinose operon studied by gel electrophoresis DNA binding assay.

@article{Hendrickson1984RegulationOT,
  title={Regulation of the Escherichia coli L-arabinose operon studied by gel electrophoresis DNA binding assay.},
  author={William G Hendrickson and Robert Schleif},
  journal={Journal of molecular biology},
  year={1984},
  volume={178 3},
  pages={611-28}
}
DNA binding properties of the proteins required for induction of the Escherichia coli L-arabinose operon were measured using a polyacrylamide gel electrophoresis assay. The mechanisms of induction and repression were studied by observing the multiple interactions of RNA polymerase, cyclic AMP receptor protein and araC protein with short DNA fragments containing either the araC or araBAD promoter regions. These studies show that binding of araC protein to the operator site, araO1, directly… CONTINUE READING

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