Regulation of the Drosophila bHLH-PAS protein Sima by hypoxia: functional evidence for homology with mammalian HIF-1 alpha.

@article{Bacon1998RegulationOT,
  title={Regulation of the Drosophila bHLH-PAS protein Sima by hypoxia: functional evidence for homology with mammalian HIF-1 alpha.},
  author={Nigel Bacon and Pablo Wappner and Joseph F. O'Rourke and Stephen Marshall Bartlett and Ben-Zion Shilo and Christopher W Pugh and Peter J Ratcliffe},
  journal={Biochemical and biophysical research communications},
  year={1998},
  volume={249 3},
  pages={811-6}
}
Hypoxia inducible factor-1 (HIF-1) is a heterodimeric complex of two basic-helix-loop-helix proteins of the PAS family which is critical for oxygen-dependent expression of many mammalian genes. Regulation is mediated by the alpha subunit (HIF-1 alpha) and sequences from HIF-1 alpha can confer hypoxia-inducible activity on a Ga14 fusion protein. To analyse conservation of this system of gene regulation between Drosophila and mammalian cells we constructed Ga14 fusions with a series of Drosophila… CONTINUE READING