Corpus ID: 23986022

Regulation of sulfated glycosaminoglycan production by prostaglandin E2 in cultured lung fibroblasts.

  title={Regulation of sulfated glycosaminoglycan production by prostaglandin E2 in cultured lung fibroblasts.},
  author={Joel Karlinsky and Ronald H. Goldstein},
  journal={The Journal of laboratory and clinical medicine},
  volume={114 2},
Prostaglandin E2 (PGE2) has been shown to increase the synthesis of hyaluronic acid in cultured fibroblasts by increasing the activity of hyaluronate synthetase, a group of plasma membrane-bound synthetic enzymes. We examined whether PGE2 also increased the activity of those enzyme systems involved in the synthesis of sulfated glycosaminoglycan in the human embryonic lung fibroblast. Exposure of cells to PGE2 resulted in dose-dependent increases in glucosamine incorporation into all sulfated… Expand
Basic fibroblast growth factor-induced glycosaminoglycan production in cultured vascular endothelial cells results from enhanced protein synthesis mediated by the lipoxygenase pathway.
It is suggested that bFGF may increase the number of GAG chains as a result of enhanced protein synthesis including xylosyl transferase through the lipoxygenase pathway of arachidonic acid metabolism in vascular endothelial cell layer. Expand
Enhanced prostaglandin biosynthesis in human gingival fibroblasts isolated from patients treated with phenytoin.
  • T. Modéer, I. Andurén, U. Lerner
  • Medicine
  • Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
  • 1992
The results indicate that PHT medication results in an upregulation of prostanoid formation in gingival fibroblasts partly due to an increased phospholipase A2 activity and partly dueto an increased cyclooxygenase activity. Expand
Binding of interleukin-8 to heparan sulfate and chondroitin sulfate in lung tissue.
IL-8-glycosaminoglycan interactions determine the location where IL-8 binds in lung tissue and provides a site for the dimerization of IL-9, which increases the local concentration of IL -8 in the lungs. Expand
The phenytoin metabolite p-HPPH upregulates prostaglandin biosynthesis in human gingival fibroblasts challenged to interleukin-1.
The results indicate that treatment with p-HPPH results in upregulation of prostaglandin synthesis in gingival fibroblasts challenged to IL-1 or TNF alpha at the level of phospholipase A2. Expand
Role of fatty acids and eicosanoids in modulating proteoglycan metabolism in endothelial cells.
The focus of this review is on fatty acids and eicosanoids as they may alter proteoglycan metabolism of vascular tissues and in particular of the endothelium. Expand
Effect of phenytoin on interleukin‐1β production in human gingival fibroblasts challenged to tumor necrosis factor αin vitro
Effects and interaction of tumor necrosis factor alpha (TNF alpha) and the antiepileptic drug phenytoin (PHT) on interleukin-1 beta (IL-1 beta) production as well as on prostaglandin E2 (PGE2)Expand
Phenytoin potentiates interleukin‐1‐induced prostaglandin biosynthesis in human gingival fibroblasts
The results indicate that treatment with PHT results in upregulation of prostaglandin biosynthesis in gingival fibroblasts challenged with IL‐1 or TNFα, at least partly due to enhanced level of phospholipase A2 activity. Expand
Role of Peritoneal Mesothelial Cells and Fibroblasts in the Synthesis of Hyaluronan during Peritoneal Dialysis
Although effluent dialysates contain factors that stimulate the production of HA by mesothelium, there is weak correlation between that stimulatory effect and the actual HA concentration in the dialysate, which might suggest low “responsiveness” of the membrane. Expand
Explant culture of rabbit tracheobronchial epithelium: structure and prostaglandin metabolism.
It is concluded that rabbit tracheal explants cultured in vitro under the above conditions maintain cellular differentiation, in situ three-dimensional organization, and PGE2 synthetic pathways over several days in culture. Expand
Elucidating interactions between the dermal fibroblast phenotype, inflammatory signals and extra-cellular matrix components
The role of prostaglandin E2 in the regulation of fibroblast activities relevant to restoration of tissue structure and function is addressed and exogenous PGE2 decreases the migratory and contractile potential of dermal fibroblasts through destabilization of the actin cytoskeleton and inhibits endogenous collagen synthesis. Expand