Regulation of sucrase-isomaltase gene expression along the crypt-villus axis of rat small intestine.

@article{Traber1990RegulationOS,
  title={Regulation of sucrase-isomaltase gene expression along the crypt-villus axis of rat small intestine.},
  author={Peter G. Traber},
  journal={Biochemical and biophysical research communications},
  year={1990},
  volume={173 3},
  pages={
          765-73
        }
}
The expression of sucrase-isomaltase mRNA was investigated along the crypt-villus axis of rat small intestine using differentially isolated cells and in situ hybridization. A partial rat sucrase-isomaltase cDNA was cloned which coded for a protein that was predicted to be 88% homologous to those encoded by the rabbit and human cDNAs. Southern blot analysis of rat genomic DNA indicated that the cDNA hybridized to a single gene. Northern blots of RNA extracted from subpopulations of intestinal… CONTINUE READING

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