Regulation of replicon size in Xenopus egg extracts.

@article{Walter1997RegulationOR,
  title={Regulation of replicon size in Xenopus egg extracts.},
  author={Johannes C. Walter and John W Newport},
  journal={Science},
  year={1997},
  volume={275 5302},
  pages={993-5}
}
Once a specific number of cells have been produced in the early Xenopus laevis embryo, replicon size during the S phase of the cell cycle increases. Here, it is reported that similar increase in replicon size occurred when the concentration of nuclei in replication-competent Xenopus egg extracts exceeded a critical threshold. In this system, the origin recognition complex (ORC) did not become stoichiometrically limiting for initiation, and similar amounts of this complex bound to chromatin… CONTINUE READING