Regulation of polyhydroxyalkanoate synthases (phaC1 and phaC2) gene expression in Pseudomonas corrugata

@article{Conte2006RegulationOP,
  title={Regulation of polyhydroxyalkanoate synthases (phaC1 and phaC2) gene expression in Pseudomonas corrugata},
  author={Enrico Conte and Vittoria Catara and Sebastiana Greco and Marcella Russo and Rossana Alicata and Luciana Strano and Alessandro Lombardo and Silvia Di Silvestro and Antonino F. Catara},
  journal={Applied Microbiology and Biotechnology},
  year={2006},
  volume={72},
  pages={1054-1062}
}
In this study we examined polyhydroxyalkanoate (PHA) synthases phaC1 and phaC2 gene expression in two strains of Pseudomonas corrugata (Pc) grown in a minimum mineral medium with related (oleic acid and octanoate) or unrelated (glucose) carbon sources. Analysis of transcription was performed by Northern blot and conventional reverse transcriptase (RT) polymerase chain reaction (PCR). In addition, we developed a RT-real-time PCR method to quantitatively evaluate phaC1 Pc and phaC2 Pc gene… CONTINUE READING