Regulation of plasmid DNA synthesis: Isolation and characterization of copy number mutants of miniR6-5 and miniF plasmids

@article{Ely1981RegulationOP,
  title={Regulation of plasmid DNA synthesis: Isolation and characterization of copy number mutants of miniR6-5 and miniF plasmids},
  author={Susan Ely and W. L. Staudenbauer},
  journal={Molecular and General Genetics MGG},
  year={1981},
  volume={181},
  pages={29-35}
}
Copy number (Cop) mutants of miniR6-5 and miniF plasmid derivatives containing a β-lactamase gene were isolated by selection for increased ampicillin-resistance. Mutants which exhibited an increased copy number and a reduced incompatibility response as compared to the respective parent mini-plasmid were obtained from both miniR6-5 and miniF. Characterization of these mutant plasmids has provided the first description of the replicative properties of miniF Cop mutants, and has also facilitated a… CONTINUE READING