Regulation of nitrogen fixation in Azorhizobium caulinodans: identification of a fixK-like gene, a positive regulator of nifA.

@article{Kaminski1991RegulationON,
  title={Regulation of nitrogen fixation in Azorhizobium caulinodans: identification of a fixK-like gene, a positive regulator of nifA.},
  author={Pierre Alexandre Kaminski and Karine Mandon and Fabrizio Arigoni and Nicole Desnoues and Claudine Elmerich},
  journal={Molecular microbiology},
  year={1991},
  volume={5 8},
  pages={
          1983-91
        }
}
The nucleotide sequence of a 1 kb fragment upstream of Azorhizobium caulinodans fixL was established. An open reading frame of 744 bp was identified as a fixK homologue. A kanamycin cartridge was inserted into the cloned fixK-like gene and recombined into the host genome. The resulting mutant was Nif-Fix-, suggesting that FixK was required for nitrogen fixation both in symbiotic conditions and in the free-living state. Using a pfixK-lacZ fusion, the FixLJ products were shown to control the… CONTINUE READING

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