Regulation of nitrogen fixation in Azorhizobium caulinodans: identification of a fixK‐like gene, a positive regulator of nifA

@article{Kaminski1991RegulationON,
  title={Regulation of nitrogen fixation in Azorhizobium caulinodans: identification of a fixK‐like gene, a positive regulator of nifA},
  author={P. Kaminski and K. Mandon and F. Arigoni and N. Desnoues and C. Elmerich},
  journal={Molecular Microbiology},
  year={1991},
  volume={5}
}
The nucleotide sequence of a 1 kb fragment upstream of Azorhizobium caulinodans fixL was established. An open reading frame of 744 bp was identified as a fixK homologue. A kanamycin cartridge was inserted into the cloned fixK‐1ike gene and recombined Into the host genome. The resulting mutant was Nif− Fix− suggesting that FixK was required for nitrogen fixation both in symbiotic conditions and in the free‐living state. Using a pfixK–lacZ fusion, the FixLJ products were shown to control the… Expand
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