Regulation of neurabin I interaction with protein phosphatase 1 by phosphorylation.

@article{McAvoy1999RegulationON,
  title={Regulation of neurabin I interaction with protein phosphatase 1 by phosphorylation.},
  author={Thomas McAvoy and P B Allen and H Obaishi and Hiroshi Nakanishi and Yoshio Takai and Paul Greengard and Angus C. Nairn and Hugh C Hemmings},
  journal={Biochemistry},
  year={1999},
  volume={38 39},
  pages={12943-9}
}
Neurabin I is a brain-specific actin-binding protein. Here we show that neurabin I binds protein phosphatase 1 (PP1) and inhibits PP1 activity. Neurabin I interacted with PP1alpha in an overlay assay, in yeast two-hybrid interaction analysis, and in coprecipitation and co-immunoprecipitation experiments. Neurabin I also copurified with both the alpha and gamma isoforms of PP1. A glutathione S-transferase (GST)-neurabin I fusion protein (residues 318-661) containing the putative PP1 binding… CONTINUE READING