Regulation of ion channel function by the host lipid bilayer examined by a stopped-flow spectrofluorometric assay.

Abstract

To examine the function of ligand-gated ion channels in a defined membrane environment, we developed a robust sequential-mixing fluorescence-based stopped-flow assay. Channel activity is determined using a channel-permeable quencher (e.g., thallium, Tl(+)) of a water-soluble fluorophore (8-aminonaphthalene-1,3,6-trisulfonic acid) encapsulated in large… (More)
DOI: 10.1016/j.bpj.2014.01.027

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Cite this paper

@article{Rusinova2014RegulationOI, title={Regulation of ion channel function by the host lipid bilayer examined by a stopped-flow spectrofluorometric assay.}, author={Radda Rusinova and Dorothy Kim and Crina M Nimigean and Olaf S Andersen}, journal={Biophysical journal}, year={2014}, volume={106 5}, pages={1070-8} }