Regulation of immediate-early gene transcription following activation of Gα(q)-coupled designer receptors.

@article{Kaufmann2013RegulationOI,
  title={Regulation of immediate-early gene transcription following activation of Gα(q)-coupled designer receptors.},
  author={Anke Kaufmann and Anja Keim and Gerald Thiel},
  journal={Journal of cellular biochemistry},
  year={2013},
  volume={114 3},
  pages={681-96}
}
G-protein coupled designer receptors that are specifically activated by designer drugs have been developed. Here, we have analyzed the regulation of gene transcription following activation of Gα(q)-coupled designer receptor (Rα(q)). Stimulation of human embryonic kidney (HEK) 293 cells expressing Rα(q) with clozapine-N-oxide (CNO), a pharmacologically inert compound, induced the expression of biologically active Egr-1, a zinc finger transcription factor. Expression of a dominant-negative mutant… CONTINUE READING