Regulation of human eosinophil viability, density, and function by granulocyte/macrophage colony-stimulating factor in the presence of 3T3 fibroblasts

@article{Owen1987RegulationOH,
  title={Regulation of human eosinophil viability, density, and function by granulocyte/macrophage colony-stimulating factor in the presence of 3T3 fibroblasts},
  author={William F. Owen and Marc E Rothenberg and David S. Silberstein and Judith C. Gasson and Richard L. Stevens and K Frank Austen and Roy J. Soberman},
  journal={The Journal of Experimental Medicine},
  year={1987},
  volume={166},
  pages={129 - 141}
}
Normodense human peripheral blood eosinophils were isolated under sterile conditions from the 22/23 and 23/24% interfaces and the cell pellet of metrizamide gradients. After culture for 7 d in RPMI media in the presence of 50 pM biosynthetic (recombinant) human granulocyte/macrophage colony-stimulating factor (rH GM-CSF), 43 +/- 7% (mean +/- SEM, n = 8) of the cells were viable; in the absence of rH GM-CSF, no eosinophils survived. The rH GM-CSF-mediated viability was concentration dependent… CONTINUE READING

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