Regulation of human airway ciliary beat frequency by intracellular pH.

@article{Sutto2004RegulationOH,
  title={Regulation of human airway ciliary beat frequency by intracellular pH.},
  author={Zoltan Sutto and Gregory E. Conner and Matthias Salathe},
  journal={The Journal of physiology},
  year={2004},
  volume={560 Pt 2},
  pages={519-32}
}
pHi affects a number of cellular functions, but the influence of pHi on mammalian ciliary beat frequency (CBF) is not known. CBF and pHi of single human tracheobronchial epithelial cells in submerged culture were measured simultaneously using video microscopy (for CBF) and epifluorescence microscopy with the pH-sensitive dye BCECF. Baseline CBF and pHi values in bicarbonate-free medium were 7.2 +/- 0.2 Hz and 7.49 +/- 0.02, respectively (n = 63). Alkalization by ammonium pre-pulse to pHi 7.78… CONTINUE READING