Regulation of human P2X1 promoter activity by beta helix-loop-helix factors in smooth muscle cells.

@article{Dhulipala2001RegulationOH,
  title={Regulation of human P2X1 promoter activity by beta helix-loop-helix factors in smooth muscle cells.},
  author={Prasad D K Dhulipala and Elias A. Lianos and Michael I. Kotlikoff},
  journal={Gene},
  year={2001},
  volume={269 1-2},
  pages={167-75}
}
We isolated and characterized genomic clones of the human P2X1 receptor (hP2X1) gene in an effort to understand its tissue specific expression. The hP2X1 gene contains 12 exons spanning 20 kb, with exon sizes ranging from 59 to 143 bp. A 385 bp upstream fragment promoted hP2X1 gene expression in smooth muscle (A7R5 and primary trachealis) and fibroblast (NIH3T3) cell lines, and mutation of a consensus E box sequence (CACCTG) within this fragment (-340 to -345) did not alter basal promoter… CONTINUE READING