Regulation of fibroblast cyclooxygenase synthesis by interleukin-1.

  title={Regulation of fibroblast cyclooxygenase synthesis by interleukin-1.},
  author={Amiram Raz and A A Wyche and Ned Siegel and Philip Needleman},
  journal={The Journal of biological chemistry},
  volume={263 6},
We have prepared polyclonal antiserum against sheep seminal vesicle prostaglandin H synthase (also termed cyclooxygenase) which cross-reacted with human cyclooxygenase, thereby enabling us to directly determine the synthetic rate of cyclooxygenase protein and its modulation by the monokine interleukin-1 (IL-1). Cultured human dermal fibroblast cells were labeled with [35S]methionine, and the membrane-bound cyclooxygenase was solubilized and immunoprecipitated 35S-labeled fibroblast… CONTINUE READING


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